The effect of microwave radiation on osteogenesis and osteolysis of rats in the hypergravity condition and the role of Rana sylvatica Le conte oil as a radioprotector

*Corresponding author: Dr. Zhong-Li Gao, Department of Orthopaedics, China-Japan Union Hospital of Jilin University, #126 Xiantai Street, Changchun City, Jilin Province, P.R. China, 130033. Fax: +860 43 184611598 E-mail: [email protected] Background: Microwave Thermotherapy has been gradually used in radiotherapy and it is reported that microwave radiation increases bone demineralization rate independently of temperature. Furthermore, it becomes more and more widespread that the damage caused by the synergistic effect of microwave radiation and hypergravity. The experiment was designed to investigate the effect of microwave radiation on osteogenesis and osteolysis of rats in the hypergravity condition and to observe the role of Rana sylvatica Le conte oil as a radioprotector. Materials and Methods: Wistar rats were exposed to microwave radiation of 200mW/cm2 power density and +6G hypergravity, and the concentrations of osteocalcin, calcitionin, calcium, phosphorus, and hydroxyproline and the activity of alkaline phosphatase were detected in serum. Results: The serum concentrations of osteocalcin and calcitionin and the activity of alkaline phosphatase decreased, while the calcium, phosphorus and hydroxyproline concentrations increased after the synergistic effect of microwave radiation and hypergravity. Rana sylvatica Le conte oil intervention inhibited significantly the decrease of osteocalcin concentration and restrained significantly the increases of hydroxyproline and calcium concentrations in serum. Conclusion: These results imply that the synergistic effect of microwave radiation and hypergravity inhibits osteogenesis and enhances osteolysis in rats causing bone metabolic disturbance, while the injury resulted from microwave radiation and hypergravity could be protected by intervention with Rana sylvatica Le conte oil and it provides an original direction in the investigation of radioprotectors. Iran.